关键词: PD-1, 人源化小鼠, 基因型

Abstract: Objective To explore the breeding and genotype identification of PD-1 humanized mice, and provide an animal model for further research on the efficacy evaluation of small molecule inhibitors in vivo. Method Through the construction of PD-1 humanized mice, four F1 generation mice were obtained, which were bred by mating male and female. The number and survival rate of each litter of mice were recorded and observed, and then genomic DNA was extracted from the offspring by tail clipping, the target gene fragment was amplified by PCR, and then the genotype was identified by nucleic acid electrophoresis. The F2 generation homozygous type and homozygous type were used for male and female mating, and wild type and wild type were used for male and female mating. Result The F2 generation mice were identified as wild type, homozygous type and heterozygous type. The genotype of F3 offspring obtained from homozygous and homozygous mating were all homozygous. The genotype of F3 offspring obtained from wild type and wild type mating were all wild type. Conclusion The homozygous genotype of PD-1 humanized mouse was successfully screened, and the population expansion and seed conservation were effectively carried out, providing experimental guarantee for the future application of this mouse model. 

Key words: PD-1, humanized mice, genotype